Test Details
Mnemonic
AVPCR
Ordering Code
3092120
CPT-4 Code(s)
87798
Specimen Information
Collect
Collection varies by specimen type, See specimen preparation.
Specimen Preparation
Specimen source required.
Swabs (genital, dermal, vesicle, ocular, oral, nasal, throat, rectal) in viral transport medium.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Biopsy/tissue specimens in saline or viral transport medium (Snap frozen-20°C).
Bronchoalveolar lavage/wash in sterile IATA-approved screw capped plastic container 1.0 mL (0.5 mL minimum).
CSF and body fluid undiluted in sterile IATA-approved screw capped plastic container. 1.0 mL (0.5 mL minimum).
Nasal aspirated in vacuum trap. 1.0 mL (0.5 mL minimum).
Nasal washes in IATA-approved screw-capped plastic container. 1.0 mL (0.5 mL minimum).
EDTA plasma (lavender top). 1.0 mL (0.5 mL minimum)
Bone marrow in EDTA (lavender top) 1.0 mL (0.5 mL minimum)
Urine undiluted in an IATA-approved screw-capped plastic container. 2.0 mL (1.0 mL minimum)
Stool in sterile IATA-approved screw-capped plastic container 2.0 mL (1.0 mL minimum) for liquid stool or marble -size solid stool
Swabs (genital, dermal, vesicle, ocular, oral, nasal, throat, rectal) in viral transport medium.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Biopsy/tissue specimens in saline or viral transport medium (Snap frozen-20°C).
Bronchoalveolar lavage/wash in sterile IATA-approved screw capped plastic container 1.0 mL (0.5 mL minimum).
CSF and body fluid undiluted in sterile IATA-approved screw capped plastic container. 1.0 mL (0.5 mL minimum).
Nasal aspirated in vacuum trap. 1.0 mL (0.5 mL minimum).
Nasal washes in IATA-approved screw-capped plastic container. 1.0 mL (0.5 mL minimum).
EDTA plasma (lavender top). 1.0 mL (0.5 mL minimum)
Bone marrow in EDTA (lavender top) 1.0 mL (0.5 mL minimum)
Urine undiluted in an IATA-approved screw-capped plastic container. 2.0 mL (1.0 mL minimum)
Stool in sterile IATA-approved screw-capped plastic container 2.0 mL (1.0 mL minimum) for liquid stool or marble -size solid stool
Alternate Specimen
The Laboratory Director or Supervisor must approve testing of specimens other than those listed.
Minimum Volume
Determined by specimen type
Transport Temperature
Refrigerated
Rejection Criteria
Rectal swabs preserved in formalin, SAF, or PVA
Urine in Grey-Top (boric acid) urine vacutainers
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media
Specimens in bacteriological blood culture media
Dry swabs, calcium alginate swabs, and swabs in gel transports
Culturettes older than 24 hours
Specimens received in non-sterile or leaking containers
Serum
Whole blood
Urine in Grey-Top (boric acid) urine vacutainers
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media
Specimens in bacteriological blood culture media
Dry swabs, calcium alginate swabs, and swabs in gel transports
Culturettes older than 24 hours
Specimens received in non-sterile or leaking containers
Serum
Whole blood
Stability
Room temperature: 3 days
Refrigerated: 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months
Refrigerated: 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months
Methodology
Polymerase chain reaction (PCR). This method is genus specific and cannot distinguish adenoviral subtypes. The analytical sensitivity of this assay is 200 DNA copies/mL.
Reference Range
Not detected
Performing Laboratory
Warde Medical Laboratory
New York DOH Approval Status
No