Test Details
Mnemonic
ALDR
Ordering Code
1003990
CPT-4 Code(s)
82088, 84244
Specimen Information
Patient Preparation
Angiotensin Converting Enzyme (ACE) Inhibitors should be avoided, if possible, for at least 48 hours prior to collection. Spironlactone (Aldactone) should be discontinued 4 - 6 weeks prior to testing. Patient should be on a normal sodium diet.
Collect
Aldosterone: Serum separator tube (SST) and
Direct Renin: Lavender EDTA
Direct Renin: Lavender EDTA
Specimen Preparation
Aldosterone:
Centrifuge, separate serum from cells and send 1.0 mL in a screw capped
plastic vial. Attach the serum label to this sample.
Direct Renin:
Immediately after collection, centrifuge lavender EDTA collection tube in a non-refrigerated centrifuge and separate plasma from cells. Send 1.5 mL plasma in screw capped plastic vial. CRITICAL FROZEN. Attach the plasma label to this sample.
Centrifuge, separate serum from cells and send 1.0 mL in a screw capped
plastic vial. Attach the serum label to this sample.
Direct Renin:
Immediately after collection, centrifuge lavender EDTA collection tube in a non-refrigerated centrifuge and separate plasma from cells. Send 1.5 mL plasma in screw capped plastic vial. CRITICAL FROZEN. Attach the plasma label to this sample.
Alternate Specimen
Aldosterone:
Serum: Red top
Direct Renin: No alternate specimen
Serum: Red top
Direct Renin: No alternate specimen
Minimum Volume
Aldosterone: 0.5 mL
Direct Renin: 1.0 mL
Direct Renin: 1.0 mL
Transport Temperature
Serum (Aldosterone): Refrigerated
Plasma (Direct Renin): CRITICAL FROZEN
Plasma (Direct Renin): CRITICAL FROZEN
Rejection Criteria
Aldosterone sample: plasma, gross hemolysis, gross lipemia, incorrect sample identification.
Direct Renin: Hemolysis, gross lipemia, icteria, nonfrozen plasma, serum, plasma from dark blue
EDTA collection tubes, incorrect sample type identification.
Direct Renin: Hemolysis, gross lipemia, icteria, nonfrozen plasma, serum, plasma from dark blue
EDTA collection tubes, incorrect sample type identification.
Stability
Aldosterone:
Room temperature: Unacceptable
Refrigerated: 5 days
Frozen: 30 days
Renin:
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days
Room temperature: Unacceptable
Refrigerated: 5 days
Frozen: 30 days
Renin:
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days
Methodology
Aldosterone: Chemiluminescence
Direct Renin: Chemiluminescence
Ratio: Calculation
Direct Renin: Chemiluminescence
Ratio: Calculation
Reference Range
Aldosterone: Upright: ≤39.2 ng/dL
Supine: ≤23.2 ng/dL
Aldosterone/Direct Renin Ratio: 0.1 - 3.7
Renin: 3.1 - 57.1 pg/mL
Supine: ≤23.2 ng/dL
Aldosterone/Direct Renin Ratio: 0.1 - 3.7
Renin: 3.1 - 57.1 pg/mL
Performing Laboratory
Warde Medical Laboratory
New York DOH Approval Status
Yes