Test Details
Mnemonic
ANGLM
Ordering Code
3600286
CPT-4 Code(s)
81331. Add 81265 for fetal specimens.
Specimen Information
Collect
Lavender EDTA
Specimen Preparation
Send 3.0 mL whole blood.
Alternate Specimen
Fetal Specimen:
Collect:
Two T-25 flasks at 80 percent confluent of cultured amniocytes AND Maternal Whole Blood
Specimen: Lavender (EDTA), pink (K2EDTA), or yellow (ACD solution A) Fetal Specimens will
require MCCFETAL testing to be added on by ARUP, and additional charges will apply.
Specimen Preparation:
Cultured Amniocytes: Fill flasks with culture media. Transport two T-25 flasks at 80 percent
confluent of cultured amniocytes filled with culture media. Backup cultures must be retained at the client's institution until testing is complete. If ARUP receives a sample below the minimum
confluence, CG GRW&SND (0040182) will be added on by ARUP, and additional charges will apply.
If clients are unable to culture specimens, CG GRW&SND should be added to initial order.
Maternal Whole Blood Specimen: Transport 3 mL whole blood (Min: 1 mL)
Transport Temperature:
Cultured Amniocytes: CRITICAL ROOM TEMPERATURE. Must be received within 48 hours of shipment due to viability Maternal Whole Blood Specimen: Refrigerated. Also acceptable:
Ambient.
Collect:
Two T-25 flasks at 80 percent confluent of cultured amniocytes AND Maternal Whole Blood
Specimen: Lavender (EDTA), pink (K2EDTA), or yellow (ACD solution A) Fetal Specimens will
require MCCFETAL testing to be added on by ARUP, and additional charges will apply.
Specimen Preparation:
Cultured Amniocytes: Fill flasks with culture media. Transport two T-25 flasks at 80 percent
confluent of cultured amniocytes filled with culture media. Backup cultures must be retained at the client's institution until testing is complete. If ARUP receives a sample below the minimum
confluence, CG GRW&SND (0040182) will be added on by ARUP, and additional charges will apply.
If clients are unable to culture specimens, CG GRW&SND should be added to initial order.
Maternal Whole Blood Specimen: Transport 3 mL whole blood (Min: 1 mL)
Transport Temperature:
Cultured Amniocytes: CRITICAL ROOM TEMPERATURE. Must be received within 48 hours of shipment due to viability Maternal Whole Blood Specimen: Refrigerated. Also acceptable:
Ambient.
Minimum Volume
1.0 mL
Transport Temperature
Refrigerated
Rejection Criteria
Direct chorionic villus sampling (direct CVS), direct amniotic fluid (direct AF), plasma, serum, and
tissue (FFPE)
tissue (FFPE)
Stability
Nonfetal Specimens, Whole Blood:
Room temperature: 7 days
Refrigerated: 30 days
Frozen: Unacceptable
Fetal Specimens, Cultured Amniocytes:
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Maternal Whole Blood Specimen:
Room temperature: 7 days
Refrigerated: 30 days
Frozen: Unacceptable
Room temperature: 7 days
Refrigerated: 30 days
Frozen: Unacceptable
Fetal Specimens, Cultured Amniocytes:
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Maternal Whole Blood Specimen:
Room temperature: 7 days
Refrigerated: 30 days
Frozen: Unacceptable
Methodology
Qualitative/Methylation-Specific Multiplex Ligation-Dependent Probe Amplification (MS-MLPA)
Reference Range
Interpretive report
Performing Laboratory
ARUP
New York DOH Approval Status
No