Test Details
Mnemonic
PBKQN
Ordering Code
3092740
CPT-4 Code(s)
87799
Specimen Information
Collect
Lavender EDTA
Specimen Preparation
Centrifuge and separate plasma from cells within 8 hours of collection. Send 2.0 mL plasma frozen in a screw capped plastic vial. Dedicated specimens are required. Specimens used in other assays will not be tested.
Minimum Volume
1.0 mL
Transport Temperature
Frozen
Rejection Criteria
Serum specimens, heparinized specimens, specimens in any gel-based separation media, shared specimens, specimens submitted to repeated freeze-thaw cycles, specimens received in non-sterile or leaking containers, specimens that do not meet the storage/handling conditions criteria above
Stability
Room temperature: 8 hours
Refrigerated: 3 days
Frozen (-20°C): 30 days
Frozen (-70°C): 6 months
Refrigerated: 3 days
Frozen (-20°C): 30 days
Frozen (-70°C): 6 months
Methodology
This test utilizes a polymerase chain reaction to amplify sequences from the VP1 regions of the BK virus genome. Real-time detection and quantification are used to determine the viral copy number. The analytical measurement range is 125 to 5 million copies/mL (2.10 to 6.70 log(10) copies/mL). The qualitative limit of detection is 100 copies/mL (2.00 log(10) copies/mL).
Specimens reported as "DETECTED" but <125 copies/mL contain detectable levels of BK virus DNA but the viral load is below the limit of quantitation. A "NOT DET" (NOT detected result does not rule out infection.
Specimens reported as "DETECTED" but <125 copies/mL contain detectable levels of BK virus DNA but the viral load is below the limit of quantitation. A "NOT DET" (NOT detected result does not rule out infection.
Reference Range
BKV QUAL: Not detected (NOT DET)
BKV QUANT: <125 copies/mL
Log BKV: <2.10 log(10) copies/mL
BKV QUANT: <125 copies/mL
Log BKV: <2.10 log(10) copies/mL
Performing Laboratory
Warde Medical Laboratory
New York DOH Approval Status
No