Test Details
Mnemonic
UBKQN
Ordering Code
3092700
CPT-4 Code(s)
87799
Specimen Information
Collect
Random urine
Specimen Preparation
Send 2.0 mL urine undiluted and frozen in a sterile screw capped plastic container. Dedicated specimens are required. Specimens used in other assays will not be tested.
Minimum Volume
1.0 mL
Transport Temperature
Frozen
Rejection Criteria
Shared specimens, specimens submitted to repeated freeze-thaw cycles, urine in Grey top (boric acid) urine Vacutainers, specimens received in non-sterile or leaking containers, specimens that do not meet the storage/handling conditions criteria above
Stability
Room temperature: 8 hours
Refrigerated: 3 days
Frozen (-20°C): 30 days
Frozen (-70°C): 6 months
Refrigerated: 3 days
Frozen (-20°C): 30 days
Frozen (-70°C): 6 months
Methodology
This test utilizes a polymerase chain reaction to amplify sequences from the VP1 regions of the BK virus genome. Real-time detection & quantification are used to determine the viral copy number. The analytical measurement range is 125 to 5 million copies/mL with an extended range up to 50 billion copies/mL with dilution (2.10 to 10.70 log(10 copies/mL). The qualitative limit to detection is 100 copies/mL (2.00 log(10) copies/mL). Specimens reported as "DETECTED" byt <125 copies/mL contain detectable levels of BK virus DNA but the viral load is below the limit of quantitation. A "Not detected" result does not rule out infection.
Reference Range
BKV QUAL: Not detected (NOT DET)
BKV QUANT: <125 copies/mL
Log BKV: <2.10 log(10) copies/mL
BKV QUANT: <125 copies/mL
Log BKV: <2.10 log(10) copies/mL
Performing Laboratory
Warde Medical Laboratory
New York DOH Approval Status
No