Test Details



Ordering Code


CPT-4 Code(s)


Specimen Information


Swab specimen - See specimen preparation for acceptable samples

Specimen Preparation

Swab specimens (endocervix, endourethral, conjunctiva, rectal, peritoneal, nasopharyngeal), throat in viral transport medium. Send refrigerated. Specimen source is required.

Alternate Specimen

Place 2.0 mL (1.0 mL minimum) cul-de-sac fluids undiluted in a sterile screw capped container.
1) Nasal aspirates in vacuum trap, 1.0 mL (0.5 mL minimum)
2) Nasal wash in an IATA-approved sterile screw-capped plastic container, 2.0 mL (1.0 mL minimum)
3) Bronchoalveolar lavage/wash in an IATA-approved sterile, screw capped plastic container, 2.0 mL (1.0 mL minimum).
4) Fallopian and/or uterine tissue specimens in saline or viral transport medium (snap frozen -20°C)
The Laboratory Director or Supervisor must approve testing of specimens other than those listed above.

Minimum Volume

Determined by specimen type

Transport Temperature

Varies, See stability

Rejection Criteria

Urine specimens are not acceptable for culture (See chlamydia by TMA). Rectal swabs or stool preserved in formalin, SAF, or PVA. Specimens in Amplicor, EIA, Gen-Probe, or Probetec Transport media. Specimens in bacterial transport media, Stewart medium (Culturette). Specimens in bacteriological blood culture media. Dry swabs, wooden swabs, calcium alginate swabs and swabs in gel transports. Specimens received in nonsterile or leaking containers.


Room temperature: 8 hours
Refrigerated: 7 days
Frozen (-20°C): Unacceptable (except tissue)
Frozen (-70°C): (tissue) 30 days


Centrifugation-enhanced (shell vial) culture with culture confirmation by monoclonal antibody staining. This procedure uses an anti-LPS monoclonal antibody and it cannot distinguish C. trachomatis from other Chlamydia species.

Reference Range


Performing Laboratory

Warde Medical Laboratory