Test Details
Mnemonic
CMVQA
Ordering Code
3092501
CPT-4 Code(s)
87497
Specimen Information
Collect
Lavender EDTA
Specimen Preparation
Centrifuge and separate plasma from cells within 24 hours of collection. Send 2.0 mL plasma refrigerated in a screw capped plastic vial. Dedicated specimens are required. Specimens used in other assays will not be tested.
Minimum Volume
1.0 mL
Transport Temperature
Refrigerated
Rejection Criteria
Whole Blood Specimens (plasma must be separated prior to receipt), specimens collected in any gel-based plasma-separation media, shared specimens, specimens that do not meet the collection storage/handling conditions criteria above
Stability
Room temperature: 24 hours
Refrigerated: 5 days
Frozen: 30 days
Refrigerated: 5 days
Frozen: 30 days
Methodology
This test uses the polymerase chain reaction to amplifiy conserved regions of the cytomegalovirus (CMV) UL34 and UL80.5 genes. Real-time detection and quantification are used to determine the viral concentration. The analytical measurement range is 50-156 million IU/mL (1.70 to 8.9 log 10 IU/mL). The qualitative limit of detection is 31 IU/mL (1.49 log 10 IU/mL) compared to the WHO International Standard.
Specimens reported as POSITIVE but <50 IU/mL contain detectable levels of CMV DNA but the viral load is below the limit of quantitation. A negative result does not rule out infection.
Specimens reported as POSITIVE but <50 IU/mL contain detectable levels of CMV DNA but the viral load is below the limit of quantitation. A negative result does not rule out infection.
Reference Range
Not Detected
<50 IU/mL
<1.70 log10 IU/mL
<50 IU/mL
<1.70 log10 IU/mL
Performing Laboratory
Warde Medical Laboratory
New York DOH Approval Status
Yes