Test Details
Mnemonic
CYPCR
Ordering Code
3091950
CPT-4 Code(s)
87496
Specimen Information
Collect
Variable specimen types
Specimen Preparation
Specimen source required. Requires dedicated sample.
Swab specimens (ocular, throat, rectal, mouth/oral) in viral transport medium.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Biopsy/tissue specimens in saline or viral transport medium (Snap frozen -20°C).
Bronchoalveolar lavage/wash in sterile, leak-proof container, 1.0 mL (0.5 mL minimum).
Sputum Undiluted in sterile, leak-proof container, 1.0 mL (0.5 mL minimum)
CSF and body fluids undiluted in sterile, leak-proof container, 1.0 mL (0.5 mL minimum)
Nasal washes in sterile, leak proof container, 1.0 mL (0.5 mL minimum)
Urine undiluted in sterile, leak-proof container, 2.0 mL (1.0 mL minimum)
Blood in EDTA (lavender top) tube, 2.0 mL (1.0 mL minimum)
EDTA plasma (lavender top), 1.0 mL (0.5 mL minimum)
Bone Marrow in EDTA, 1.0 mL (0.5 mL minimum)
Amniotic Fluid, 1.0 mL (0.5 mL minimum) in sterile, leak-proof container
Swab specimens (ocular, throat, rectal, mouth/oral) in viral transport medium.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Biopsy/tissue specimens in saline or viral transport medium (Snap frozen -20°C).
Bronchoalveolar lavage/wash in sterile, leak-proof container, 1.0 mL (0.5 mL minimum).
Sputum Undiluted in sterile, leak-proof container, 1.0 mL (0.5 mL minimum)
CSF and body fluids undiluted in sterile, leak-proof container, 1.0 mL (0.5 mL minimum)
Nasal washes in sterile, leak proof container, 1.0 mL (0.5 mL minimum)
Urine undiluted in sterile, leak-proof container, 2.0 mL (1.0 mL minimum)
Blood in EDTA (lavender top) tube, 2.0 mL (1.0 mL minimum)
EDTA plasma (lavender top), 1.0 mL (0.5 mL minimum)
Bone Marrow in EDTA, 1.0 mL (0.5 mL minimum)
Amniotic Fluid, 1.0 mL (0.5 mL minimum) in sterile, leak-proof container
Alternate Specimen
The Laboratory Director or Supervisor must approve testing of specimens other than those listed.
Minimum Volume
Determined by specimen type
Transport Temperature
Varies by specimen type, see stability
Rejection Criteria
Rectal swabs preserved in formalin, SAF, or PVA
Urine in Grey-Top (boric acid) urine Vacutainers
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media
Specimens in bacteriological blood culture media
Dry swabs, calcium alginate swabs, and swabs in gel transports
Culturettes older than 24 hours
Stool
Serum
Specimens received in non-sterile or leaking containers will not be tested
Urine in Grey-Top (boric acid) urine Vacutainers
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media
Specimens in bacteriological blood culture media
Dry swabs, calcium alginate swabs, and swabs in gel transports
Culturettes older than 24 hours
Stool
Serum
Specimens received in non-sterile or leaking containers will not be tested
Stability
Room temperature (18-25°): 3 days
Refrigerated (2-8°C): 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months
Refrigerated (2-8°C): 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months
Methodology
This test utilizes the polymerase chain reaction to amplify a 60 base pair region of the cytomegalovirus (CMV) DNA polymerase gene. Real-time amplification and detection are used to determine if the specimen is positive or negative for CMV. A negative result does not rule out infection. The analytical sensitivity of this assay is 600 copies/mL.
Reference Range
Not detected
Performing Laboratory
Warde Medical Laboratory
New York DOH Approval Status
No