Test Details
Mnemonic
EGFRA
Ordering Code
3401056
CPT-4 Code(s)
81325
Specimen Information
Collect
Lung tissue biopsy, formalin-fixed paraffin-embedded block or charged/+slides from formalin-fixed paraffin embedded tissue.
Specimen Preparation
Formalin fixed paraffin embedded tissue block sent at room temperature.
FFPE tissue block Preparation: Specimen must be fixed in 10% neutral buffered formalin. Fixation between 6 and 48 hours is recommended.
Slide Preparation: Cut one 3-5 microns tissue section for H&E stain. Cut 3-5 (10 microns) sections on charged regular glass slides for macrodissection. The slide should not be baked or melted. A new blade must be used for cutting each paraffin block to prevent cross contamination.
FFPE tissue block Preparation: Specimen must be fixed in 10% neutral buffered formalin. Fixation between 6 and 48 hours is recommended.
Slide Preparation: Cut one 3-5 microns tissue section for H&E stain. Cut 3-5 (10 microns) sections on charged regular glass slides for macrodissection. The slide should not be baked or melted. A new blade must be used for cutting each paraffin block to prevent cross contamination.
Alternate Specimen
Slide: 8 (5 minimum) unstained charged (+) slides.
Minimum Volume
5 unstained charged (+) slides
Transport Temperature
Room temperature
Rejection Criteria
Fresh tissue, non-fixed tissue, frozen tissue
Stability
Room temperature: 5 years
Refrigerated: 5 years
Frozen: Unacceptable
Refrigerated: 5 years
Frozen: Unacceptable
Methodology
Next Generation Sequencing, Fluorescence in situ Hybridization (FISH)
Reference Range
EGFR Mutation: Not detected
FISH, ALK 2p23 Rearrangement: See report
FISH, ALK 2p23 Rearrangement: See report
Performing Laboratory
Quest
New York DOH Approval Status
Yes