Test Details

Mnemonic

EGFR

Ordering Code

3423400

CPT-4 Code(s)

81235

Specimen Information

Collect

Formalin fixed paraffin embedded tissue

Specimen Preparation

Formalin fixed paraffin embedded tissue block sent at room temperature.

Alternate Specimen

Slide: 8 (4 minimum) unstained charged (+) slides. Cut one 3 - 5 microns tissue section for H & E stain. Cut 3 - 5 (10 microns) sections on non-charged regular glass slides for microdissection. The slide should not be baked or melted. A new blade must be used for each paraffin block, for cutting to prevent cross contamination.

Transport Temperature

Room temperature

Rejection Criteria

Thawed tissue, fresh tissue, non-fixed tissue, frozen tissue

Stability

Block:
Room temperature: 5 years
Refrigerated: 5 years
Frozen: Unacceptable

Methodology

Polymerase Chain Reaction and Sequencing
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Reference Range

EGFR Mutation: Not detected
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Performing Laboratory

Quest SJC
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