Test Details

Mnemonic

EBVQN

Ordering Code

3000071

CPT-4 Code(s)

87799

Specimen Information

Collect

Lavender EDTA

Specimen Preparation

Centrifuge and separate plasma from cells within 6 hours of collection. Send 2.0 mL plasma frozen in a screw capped plastic vial. Dedicated specimens are required. Specimens used in other assays will not be tested.

Minimum Volume

1.0 mL

Transport Temperature

Frozen

Rejection Criteria

Serum, heparinized plasma, whole blood, specimens collected using gel-based separation tubes, specimens submitted to repeated freeze-thaw cycles

Stability

Room temperature: 8 hours
Refrigerated: 3 days
Frozen (-20°C): 30 days
Frozen (-70°C): 6 months

Methodology

Polymerase Chain Reaction (PCR)
This test uses the polymerase chain reaction to amplify regions of the Epstein Barr Virus BLLF1 gene. Real-time detection and quantification are used to determine the viral concentration. The analytical measurement range is 500 to 5 million IU/mL (2.7 to 6.7 log (10) IU/mL). The qualitative limit of detection is 50 IU/mL (1.7 log (10) IU/mL).
Specimens reported as "DETECTED" but <500 IU/mL, contain detectable levels of EB Virus DNA but the viral load is below the limit of quantification. A "NOT DETECTED" result does not rule out infection.
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Reference Range

EBV Qualitative: Not Detected
EBV Quantitative: <500 IU/mL
Log EBV: <2.7 log(10) IU/mL
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Performing Laboratory

Warde Medical Laboratory
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