Test Details
Mnemonic
GAUCH
Ordering Code
3515000
CPT-4 Code(s)
81251, add 88235 if testing is performed on amniotic fluid or chorionic villi at additional charge
Specimen Information
The following germline genetic tests from Quest require physician attestation that patient consent has been received if ordering medical facility is located in AK, DE, FL, GA, IA, MA, MN, NV, NJ, NY, OR, SD or VT or test is performed in MA.
Collect
Lavender EDTA
Specimen Preparation
Send 4.0 mL whole blood in a screw capped plastic vial.
Alternate Specimen
Whole Blood: ACD yellow top tube, royal blue top, sodium or lithium heparin green tube.
Bone marrow: Lavender EDTA tube.
Amniotic fluid collected in a sterile plastic leak-proof container, chorionic villi collected in a
sterile tube filled with sterile culture media, cultured cells in each of two sterile T-25 flasks.
Bone marrow: Lavender EDTA tube.
Amniotic fluid collected in a sterile plastic leak-proof container, chorionic villi collected in a
sterile tube filled with sterile culture media, cultured cells in each of two sterile T-25 flasks.
Minimum Volume
Whole blood and Bone marrow: 3.0 mL, Amniotic fluid: 10.0 mL, Cultured cells: 75% confluent filled with culture media, Chorionic villi: 10.0 mg
Transport Temperature
Room temperature
Stability
Whole blood and Bone marrow:
Room temperature: 8 days
Refrigerated: 8 days
Frozen: Unacceptable
Amniotic fluid, cultured cells, chorionic villi:
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Room temperature: 8 days
Refrigerated: 8 days
Frozen: Unacceptable
Amniotic fluid, cultured cells, chorionic villi:
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Methodology
Polymerase Chain Reaction (PCR), Next Generation Sequencing
Reference Range
See report
Performing Laboratory
Quest
New York DOH Approval Status
Yes