Test Details

Mnemonic

HCVSR

Ordering Code

3001452

CPT-4 Code(s)

G0472, plus 87522 if reflexed to HCV PCR, at additional cost

Specimen Information

Collect

Serum separator tube (SST), Lavender EDTA - Both specimens required

Specimen Preparation

Centrifuge, separate serum from cells and send 1.0 mL serum refrigerated and 3.0 mL plasma frozen in screw-capped plastic vials. Serum should be labeled with HCAB label, and plasma with HCVFR label.

Alternate Specimen

Red-top or lavender EDTA plasma may be substituted for the serum specimen

Minimum Volume

Serum: 0.5 mL
Plasma: 2.5 mL

Transport Temperature

Serum: Refrigerated
Plasma: Frozen

Rejection Criteria

Gross hemolysis, gross lipemia, heparinized plasma, shared specimens, specimens subjected to repeated freeze/thaw

Stability

Serum:
Room temperature: Unacceptable
Refrigerated: 5 days
Frozen (-20°C): 14 days

Plasma:
Room temperature: Unacceptable
Refrigerated: 3 days
Frozen (-20°C): 60 days
Frozen (-70°C): 60 days

Methodology

HCV Antibody Screen Methodology: Chemiluminscence

HCV RNA, PCR Methodology: Reactive specimens are tested for HCV RNA using a real-time polymerase chain reaction (PCR) test from Abbott Molecular. The amplification target is a conserved region of the HCV genome. The lower limit of quantitation is 12 IU/mL (1.08 Log IU/mL) and the upper limit of quantitation is 100 million IU/mL (8.00 Log IU/mL). The qualitative limit of detection is 12 IU/mL (1.08 Log IU/mL). Specimens reported as "DETECTED" but <12 IU/mL contain detectable levels of Hepatitis C RNA but the viral load is below the quantitation. A "Not detected" result does not rule out infection.
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Reference Range

HCV Antibody Screen: Nonreactive
HCV RNA, Qualitative: Not detected
HCV RNA, Quantitative: <12 IU/mL
Log HCV IU/mL: <1.08 Log IU/mL
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Performing Laboratory

Warde Medical Laboratory
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