Test Details
Mnemonic
HCVQN
Ordering Code
3041400
CPT-4 Code(s)
87522
Specimen Information
Collect
Lavender EDTA
Specimen Preparation
Centrifuge and separate plasma from cells within 6 hours of collection. Send 3.0 mL plasma in a screw capped plastic vial. Dedicated specimens are required. Specimens used in other assays will not be tested.
Alternate Specimen
Serum: Red top
Minimum Volume
2.5 mL
Transport Temperature
Frozen
Rejection Criteria
All gel-based plasma separate tubes, shared specimens, specimens subjected to repeated freeze-thaw cycles, specimens that do not meet the storage/handling conditions criteria
Stability
Room temperature: Unacceptable
Refrigerated: 3 days
Frozen (-20°C): 60 days
Frozen (-70°C): 60 days
Refrigerated: 3 days
Frozen (-20°C): 60 days
Frozen (-70°C): 60 days
Methodology
This procedure utilizes a real-time Polymerase Chain Reaction (PCR) test from Abbott Molecular. The amplification target is a conserved region of the HCV genome. The lower limit of quantitation is 12 IU/mL (1.08 Log IU/mL) and the upper limit of quantitation is 100 million IU/mL (8.00 Log IU/mL).
The qualitative limit of detection is 12 IU/mL (1.08 Log IU/mL). Specimens reported as "Detected" but <12 IU/mL contain detectable levels of hepatitis C RNA but the viral load is below the limit of quantitation. A "Not detected" result does not rule out infection.
The qualitative limit of detection is 12 IU/mL (1.08 Log IU/mL). Specimens reported as "Detected" but <12 IU/mL contain detectable levels of hepatitis C RNA but the viral load is below the limit of quantitation. A "Not detected" result does not rule out infection.
Reference Range
Hepatitis C RNA: Not detected
Hepatitis C RNA, Quantitative: <12 IU/mL
LOG HCV IU/mL: <1.08 Log IU/mL
Hepatitis C RNA, Quantitative: <12 IU/mL
LOG HCV IU/mL: <1.08 Log IU/mL
Performing Laboratory
Warde Medical Laboratory
New York DOH Approval Status
Yes