Test Details
Mnemonic
AHPR
Ordering Code
3001485
CPT-4 Code(s)
80074, plus 87522 if reflexed to HCV PCR, at additional cost
Specimen Information
Performance of the HCV assay has not been established with cord blood or
neonatal specimens.
Collect
Serum separator tube (SST), Lavender EDTA - Both specimens required
Specimen Preparation
Centrifuge, separate serum from cells and send 4.0 mL serum and 3.0 mL plasma in screw capped plastic vials. Serum should be labeled with AHP label and plasma with HCVFR label.
Alternate Specimen
Red top, lavender EDTA (follow plasma collection guide for PCR)
Minimum Volume
SST: 2.0 mL serum
EDTA: 2.5 mL plasma
EDTA: 2.5 mL plasma
Transport Temperature
Serum separator tube (SST): Refrigerated
Plasma EDTA: Frozen
Plasma EDTA: Frozen
Rejection Criteria
Gross hemolysis, gross lipemia, heparin plasma
Stability
Room temperature: Unacceptable
Refrigerated: 5 days
Frozen (-20°C): Undetermined
Refrigerated: 5 days
Frozen (-20°C): Undetermined
Methodology
AHP Methodology: Chemiluminescence
HCV RNA, PCR Methodology: Reactive specimens are tested for HCV RNA using a real-time polymerase chain reaction (PCR) test from Abbott Molecular. The amplification target is a conserved region of the HCV genome. The lower limit of quantitation is 12 IU/mL (1.08 Log IU/mL) and the upper limit of quantitation is 100 million IU/mL (8.00 Log IU/mL). The qualitative limit of detection is 12 IU/mL (1.08 Log IU/mL). Specimens reported as "DETECTED" but <12 IU/mL contain detectable levels of Hepatitis C RNA but the viral load is below the quantitation. A "Not detected" result does not rule out infection.
HCV RNA, PCR Methodology: Reactive specimens are tested for HCV RNA using a real-time polymerase chain reaction (PCR) test from Abbott Molecular. The amplification target is a conserved region of the HCV genome. The lower limit of quantitation is 12 IU/mL (1.08 Log IU/mL) and the upper limit of quantitation is 100 million IU/mL (8.00 Log IU/mL). The qualitative limit of detection is 12 IU/mL (1.08 Log IU/mL). Specimens reported as "DETECTED" but <12 IU/mL contain detectable levels of Hepatitis C RNA but the viral load is below the quantitation. A "Not detected" result does not rule out infection.
Reference Range
Hepatitis A IgM Antibody Negative
Hepatitis B Surface Antigen Negative
Hepatitis B Core IgM Antibody Negative
Hepatitis C Antibody Screen Nonreactive
HCV RNA, Qualitative Not detected
HCV RNA, Quantitative <12 IU/mL
Log HCV IU/mL <1.08 Log IU/mL
See Website: Resource/Interpretation Guide
and Forms/Viral Serology Testing Guide
Hepatitis B Surface Antigen Negative
Hepatitis B Core IgM Antibody Negative
Hepatitis C Antibody Screen Nonreactive
HCV RNA, Qualitative Not detected
HCV RNA, Quantitative <12 IU/mL
Log HCV IU/mL <1.08 Log IU/mL
See Website: Resource/Interpretation Guide
and Forms/Viral Serology Testing Guide
Performing Laboratory
Warde Medical Laboratory
New York DOH Approval Status
Yes