Test Details
Mnemonic
AHPR
Ordering Code
3001485
CPT-4 Code(s)
80074, plus 87522 if reflexed to HCV PCR, at additional cost
Specimen Information
Collect
Serum separator tube (SST), Lavender EDTA - Both specimens required
Specimen Preparation
Centrifuge, separate serum from cells and send 4.0 mL serum refrigerated and 3.0 mL plasma in screw capped plastic vials. Serum should be labeled with AHP label and plasma with HCVFR label.
Alternate Specimen
Red top or lavender EDTA
Plasma may be substituted for serum
Plasma may be substituted for serum
Minimum Volume
SST: 2.0 mL serum
EDTA: 2.5 mL plasma
EDTA: 2.5 mL plasma
Transport Temperature
Refrigerated
Rejection Criteria
Grossly hemolyzed or lipemic specimens, heparinized plasma, shared specimens, specimens subjected to repeated freeze/thaw
Stability
Serum:
Room temperature: Unacceptable
Refrigerated: 5 days
Frozen (-20°C): 14 days
Plasma:
Room temperature: Unacceptable
Refrigerated: 3 days
Frozen (-20°C): 60 days
Frozen (-70°C): 60 days
Room temperature: Unacceptable
Refrigerated: 5 days
Frozen (-20°C): 14 days
Plasma:
Room temperature: Unacceptable
Refrigerated: 3 days
Frozen (-20°C): 60 days
Frozen (-70°C): 60 days
Methodology
AHP Methodology: Chemiluminescence
HCV RNA, PCR Methodology: Reactive specimens are tested for HCV RNA using a real-time polymerase chain reaction (PCR) test from Abbott Molecular. The amplification target is a conserved region of the HCV genome. The lower limit of quantitation is 12 IU/mL (1.08 Log IU/mL) and the upper limit of quantitation is 100 million IU/mL (8.00 Log IU/mL). The qualitative limit of detection is 12 IU/mL (1.08 Log IU/mL). Specimens reported as "DETECTED" but <12 IU/mL contain detectable levels of Hepatitis C RNA but the viral load is below the quantitation. A "Not detected" result does not rule out infection.
HCV RNA, PCR Methodology: Reactive specimens are tested for HCV RNA using a real-time polymerase chain reaction (PCR) test from Abbott Molecular. The amplification target is a conserved region of the HCV genome. The lower limit of quantitation is 12 IU/mL (1.08 Log IU/mL) and the upper limit of quantitation is 100 million IU/mL (8.00 Log IU/mL). The qualitative limit of detection is 12 IU/mL (1.08 Log IU/mL). Specimens reported as "DETECTED" but <12 IU/mL contain detectable levels of Hepatitis C RNA but the viral load is below the quantitation. A "Not detected" result does not rule out infection.
Reference Range
Hepatitis A IgM Antibody Negative
Hepatitis B Surface Antigen Negative
Hepatitis B Core IgM Antibody Negative
Hepatitis C Antibody Screen Nonreactive
HCV RNA, Qualitative Not detected
HCV RNA, Quantitative <12 IU/mL
Log HCV IU/mL <1.08 Log IU/mL
Hepatitis B Surface Antigen Negative
Hepatitis B Core IgM Antibody Negative
Hepatitis C Antibody Screen Nonreactive
HCV RNA, Qualitative Not detected
HCV RNA, Quantitative <12 IU/mL
Log HCV IU/mL <1.08 Log IU/mL
Performing Laboratory
Warde Medical Laboratory