Test Details
Mnemonic
HSV12
Ordering Code
3091740
CPT-4 Code(s)
87529 x 2
Specimen Information
Collect
Variable specimen types
Specimen Preparation
Specimen source required.
Swab specimens (genital, dermal, vesicle, ocular, oral, nasal, throat, rectal) in viral transport medium.
Swabs in Culturettes must be transferred to viral transport within 24 hours of collection.
Biopsy/tissue specimens in saline or viral transport medium (Snap frozen -20°C).
Bronchoalveolar lavage/wash in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).
CSF and body fluids undiluted in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).
Nasal aspirates in vacuum trap. 1.0 mL (0.5 mL minimum).
Nasal washes in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum)
Blood in EDTA (lavender top) tube. 2.0 mL (1.0 mL minimum).
EDTA plasma (lavender top). 1.0 mL (0.5 mL minimum).
Bone Marrow in EDTA. 1.0 mL (0.5 mL minimum).
Swab specimens (genital, dermal, vesicle, ocular, oral, nasal, throat, rectal) in viral transport medium.
Swabs in Culturettes must be transferred to viral transport within 24 hours of collection.
Biopsy/tissue specimens in saline or viral transport medium (Snap frozen -20°C).
Bronchoalveolar lavage/wash in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).
CSF and body fluids undiluted in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum).
Nasal aspirates in vacuum trap. 1.0 mL (0.5 mL minimum).
Nasal washes in an IATA-approved sterile screw-capped plastic container. 1.0 mL (0.5 mL minimum)
Blood in EDTA (lavender top) tube. 2.0 mL (1.0 mL minimum).
EDTA plasma (lavender top). 1.0 mL (0.5 mL minimum).
Bone Marrow in EDTA. 1.0 mL (0.5 mL minimum).
Alternate Specimen
The Laboratory Director or Supervisor must approve testing of specimens other than those listed.
Minimum Volume
Determined by specimen type
Transport Temperature
Varies with specimen type, see stability
Rejection Criteria
Rectal swabs preserved in formalin, SAF, or PVA.
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media.
Specimens in bacteriological blood culture media.
Dry swabs, calcium alginate swabs, and swabs in gel transports.
Culturettes older than 24 hours.
Specimens received in non-sterile or leaking containers.
Stool samples
Serum
Urine
E-swabs
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media.
Specimens in bacteriological blood culture media.
Dry swabs, calcium alginate swabs, and swabs in gel transports.
Culturettes older than 24 hours.
Specimens received in non-sterile or leaking containers.
Stool samples
Serum
Urine
E-swabs
Stability
Room temperature: 3 days
Refrigerated: 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months
Do not freeze whole blood or bone marrow.
Refrigerated: 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months
Do not freeze whole blood or bone marrow.
Methodology
Herpes Simplex Virus (HSV) detection is performed by a real-time PCR amplification and detection procedure. The HSV type 1 assay detects a genetic sequence at the glycoprotein G/J junction while the HSV-2 assay targets a portion of the glycoprotein G gene. The analytical sensitivity of this assay is 50 copies/mL. A negative result does not rule out infection.
Reference Range
Not detected
Performing Laboratory
Warde Medical Laboratory
New York DOH Approval Status
No