Test Details
Mnemonic
FLPCR
Ordering Code
3091830
CPT-4 Code(s)
87502
Specimen Information
Collect
Variable specimen types
Specimen Preparation
Specimen source is required.
NP and Throat swabs in viral transport medium.
If sending one sample for FLPCR and COVW (COVID testing) please send a minimum of 1.5 mL.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Bronchoalveolar lavage/wash in a sterile screw capped plastic container. Send 1.0 mL (0.5 mL minimum).
Sputum undiluted in a sterile screw capped plastic container. Send 1.0 mL (0.5 mL minimum).
Nasal aspirates in vacuum trap. 1.0 mL (0.5 mL minimum).
Nasal washes in an sterile screw capped plastic container 1.0 mL (0.5 mL minimum).
NP/OP swab in Phosphate Buffered Saline or Sterile Saline.
NP and Throat swabs in viral transport medium.
If sending one sample for FLPCR and COVW (COVID testing) please send a minimum of 1.5 mL.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Bronchoalveolar lavage/wash in a sterile screw capped plastic container. Send 1.0 mL (0.5 mL minimum).
Sputum undiluted in a sterile screw capped plastic container. Send 1.0 mL (0.5 mL minimum).
Nasal aspirates in vacuum trap. 1.0 mL (0.5 mL minimum).
Nasal washes in an sterile screw capped plastic container 1.0 mL (0.5 mL minimum).
NP/OP swab in Phosphate Buffered Saline or Sterile Saline.
Alternate Specimen
The Laboratory Director or Supervisor must approve testing of specimens other than those listed.
Minimum Volume
Determined by specimen type
Transport Temperature
Varies by specimen type, see stability
Rejection Criteria
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media.
Specimens in bacteriological blood culture media.
Dry swabs, calcium alginate swabs, and swabs in gel transports.
Culturette specimens older than 24 hours.
Specimens received in non-sterile or leaking containers.
Whole blood
Serum
Plasma
Specimens in bacteriological blood culture media.
Dry swabs, calcium alginate swabs, and swabs in gel transports.
Culturette specimens older than 24 hours.
Specimens received in non-sterile or leaking containers.
Whole blood
Serum
Plasma
Stability
Room temperature: 3 days
Refrigerated: 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months
Refrigerated: 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months
Methodology
Influenza A and Influenza B virus detection is performed by reverse transcription of Influenza A and Influenza B RNA sequences and real-time PCR amplification and detection. The Influenza A assay detects the presence of a conserved region of the matrix gene and the Influenza B assay detects a conserved region of the NS1 protein. Available nucleic acid sequence information suggest that the Influenza A test will detect all known Influenza A subtypes including avian Influenzas. However, this method cannot distinguish avian from non-avian subtypes. The analytical sensitivity of this assay is 100 copies/mL.
Reference Range
Not detected
Performing Laboratory
Warde Medical Laboratory
New York DOH Approval Status
No