Test Details

Mnemonic

FLPCR

Ordering Code

3091830

CPT-4 Code(s)

87502

Specimen Information

Collect

Variable specimen types

Specimen Preparation

Specimen source is required.
NP and Throat swabs in viral transport medium.
If sending one sample for FLPCR and COVW (COVID testing) please send a minimum of 1.5 mL.

Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Bronchoalveolar lavage/wash in a sterile screw capped plastic container. Send 1.0 mL (0.5 mL minimum).
Sputum undiluted in a sterile screw capped plastic container. Send 1.0 mL (0.5 mL minimum).
Nasal aspirates in vacuum trap. 1.0 mL (0.5 mL minimum).
Nasal washes in an sterile screw capped plastic container 1.0 mL (0.5 mL minimum).

NP/OP swab in Phosphate Buffered Saline or Sterile Saline.

Alternate Specimen

The Laboratory Director or Supervisor must approve testing of specimens other than those listed.

Minimum Volume

Determined by specimen type

Transport Temperature

Varies by specimen type, see stability

Rejection Criteria

Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media.
Specimens in bacteriological blood culture media.
Dry swabs, calcium alginate swabs, and swabs in gel transports.
Culturette specimens older than 24 hours.
Specimens received in non-sterile or leaking containers.
Whole blood
Serum
Plasma

Stability

Room temperature: 3 days
Refrigerated: 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months

Methodology

Influenza A and Influenza B virus detection is performed by reverse transcription of Influenza A and Influenza B RNA sequences and real-time PCR amplification and detection. The Influenza A assay detects the presence of a conserved region of the matrix gene and the Influenza B assay detects a conserved region of the NS1 protein. Available nucleic acid sequence information suggest that the Influenza A test will detect all known Influenza A subtypes including avian Influenzas. However, this method cannot distinguish avian from non-avian subtypes. The analytical sensitivity of this assay is 100 copies/mL.
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Reference Range

Not detected
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Performing Laboratory

Warde Medical Laboratory
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New York DOH Approval Status

No
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