Test Details

Mnemonic

JAK2Q

Ordering Code

3096210

CPT-4 Code(s)

81270

Specimen Information

Collect

Lavender EDTA

Specimen Preparation

Send 7.0 mL whole blood. Dedicated specimens are required. Specimens used in other assays will not be tested.

Alternate Specimen

Whole blood in K2EDTA (pink top) tubes are acceptable

Minimum Volume

3.0 mL

Transport Temperature

Refrigerated

Rejection Criteria

Serum, plasma, bone marrow, heparinized whole blood, tissue specimens

Stability

Room temperature: 24 hours
Refrigerated: 14 days
Frozen: Unacceptable

Methodology

This procedure uses real-time polymerase chain reaction amplification and detection to quantify the percentage of white blood cells containing the V617F (G>T at position 617) point mutation in the autoinhibitory pseudokinase domain of the JAK2 protein. The lower limit of quantitation is 0.1% (1 to 1000 cells).
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Reference Range

Percent of WBCs with V617F Mutation: <0.1%
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Performing Laboratory

Warde Medical Laboratory
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New York DOH Approval Status

No
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