Test Details
Mnemonic
MPNCP
Ordering Code
3400877
CPT-4 Code(s)
81270, 81279, 81219, 81339
Specimen Information
Collect
Lavender EDTA
Specimen Preparation
Send 4.0 mL whole blood or 3.0 mL bone marrow aspirate.
Alternate Specimen
Whole blood or bone marrow aspirate: Green sodium heparin
Fixed cell pellet: Collected in a plastic leak-proof container. Send within 7 days of fixation date.
Indicate type of fixative used. Carnoy's or other alcohol-based fixatives are acceptable.
Extracted DNA: From CLIA-certified Laboratory collected in a sterile leak-proof container.
Fixed cell pellet: Collected in a plastic leak-proof container. Send within 7 days of fixation date.
Indicate type of fixative used. Carnoy's or other alcohol-based fixatives are acceptable.
Extracted DNA: From CLIA-certified Laboratory collected in a sterile leak-proof container.
Minimum Volume
Whole blood: 3.0 mL
Bone marrow: 1.0 mL
Bone marrow: 1.0 mL
Transport Temperature
Whole blood and bone marrow: Room temperature
Cell pellet and DNA: Frozen
Cell pellet and DNA: Frozen
Rejection Criteria
Hemolysis
Stability
Room temperature: Undetermined
Refrigerated: Undetermined
Frozen: Unacceptable
Refrigerated: Undetermined
Frozen: Unacceptable
Methodology
Targeted Next Generation Sequencing
Reference Range
JAK2 V617F Mutation: Not detected
JAK2 Exon 12 Mutation: Not detected
CALR Exon 9 Mutation: Not detected
MPL Exon 10 Mutation: Not detected
JAK2 Exon 12 Mutation: Not detected
CALR Exon 9 Mutation: Not detected
MPL Exon 10 Mutation: Not detected
Performing Laboratory
Quest SJC
New York DOH Approval Status
Yes