Test Details

Mnemonic

NOPCR

Ordering Code

3092440

CPT-4 Code(s)

87798 x 2

Specimen Information

Collect

Random stool

Specimen Preparation

Source required. Send 1.0 mL stool in a sterile IATA-approved screw capped plastic container.

Alternate Specimen

Stool in Cary Blair preservative.
Swab specimens (rectal) in viral transport medium.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.

Minimum Volume

0.5 mL

Transport Temperature

Refrigerated

Rejection Criteria

Stool or rectal swabs preserved in formalin, SAF, or PVA.
Specimens received in non-sterile or leaking containers.
Stool specimens received on diapers, tisssue paper, tongue depressors, sticks or other objects. Emesis will not be tested.
Culturettes older than 24 hours.

Stability

Room temperature: 3 days
Refrigerated: 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months

Methodology

Reverse-Transcription Polymerase Chain Reaction
Norovirus virus detection was performed by reverse transcriptase polymerase chain reaction (PCR) amplification of a portion of the viral RNA polymerase gene and real-time detection using TaqMan detection probes. This method will detect genogroup 1 and genogroup 2 noroviruses. The analytical sensitivity of this assay is approximately 100 virus copies/assay. Positive results should be correlated with clinical presentation as Noroviruses are excreted in the stool for 2-3 weeks after clinical symptoms resolve. A negative result does not rule out infection.
more

Reference Range

Not detected
more

Performing Laboratory

Warde Medical Laboratory
more