Test Details
Mnemonic
NOPCR
Ordering Code
3092440
CPT-4 Code(s)
87798 x 2
Specimen Information
Collect
Random stool
Specimen Preparation
Source required. Send 1.0 mL stool in a sterile IATA-approved screw capped plastic container.
Alternate Specimen
Stool in Cary Blair preservative.
Swab specimens (rectal) in viral transport medium.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Swab specimens (rectal) in viral transport medium.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Minimum Volume
0.5 mL
Transport Temperature
Refrigerated
Rejection Criteria
Stool or rectal swabs preserved in formalin, SAF, or PVA.
Specimens received in non-sterile or leaking containers.
Stool specimens received on diapers, tisssue paper, tongue depressors, sticks or other objects. Emesis will not be tested.
Culturettes older than 24 hours.
Specimens received in non-sterile or leaking containers.
Stool specimens received on diapers, tisssue paper, tongue depressors, sticks or other objects. Emesis will not be tested.
Culturettes older than 24 hours.
Stability
Room temperature: 3 days
Refrigerated: 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months
Refrigerated: 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months
Methodology
Reverse-Transcription Polymerase Chain Reaction
Norovirus virus detection was performed by reverse transcriptase polymerase chain reaction (PCR) amplification of a portion of the viral RNA polymerase gene and real-time detection using TaqMan detection probes. This method will detect genogroup 1 and genogroup 2 noroviruses. The analytical sensitivity of this assay is approximately 100 virus copies/assay. Positive results should be correlated with clinical presentation as Noroviruses are excreted in the stool for 2-3 weeks after clinical symptoms resolve. A negative result does not rule out infection.
Norovirus virus detection was performed by reverse transcriptase polymerase chain reaction (PCR) amplification of a portion of the viral RNA polymerase gene and real-time detection using TaqMan detection probes. This method will detect genogroup 1 and genogroup 2 noroviruses. The analytical sensitivity of this assay is approximately 100 virus copies/assay. Positive results should be correlated with clinical presentation as Noroviruses are excreted in the stool for 2-3 weeks after clinical symptoms resolve. A negative result does not rule out infection.
Reference Range
Not detected
Performing Laboratory
Warde Medical Laboratory
New York DOH Approval Status
No