Test Details

Mnemonic

OSFR

Ordering Code

3802425

CPT-4 Code(s)

85557

Specimen Information

Collect

Lavender EDTA

Specimen Preparation

Collect EDTA from patient and control from a nonrelated, nonsmoking person. Send 4.0 mL whole blood in original collection tubes. Tubes must arrive refrigerated within 72 hours of collection. Label control tube clearly on outermost label "normal control". Collect sample Sunday - Thursday only.

Minimum Volume

2.0 mL

Transport Temperature

Refrigerated

Rejection Criteria

Frozen specimens, clotted specimens, gross hemolysis

Stability

Room temperature: Unacceptable
Refrigerated: 72 hours
Frozen: Unacceptable

Methodology

Osmotic Lysis
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Reference Range

≥12 months:
0.50 g/dL NaCl (unincubated): 3 - 53% hemolysis
0.60 g/dL NaCl (incubated): 14 - 74% hemolysis
0.65 g/dL NaCl (incubated): 4 - 40% hemolysis
0.75 g/dL NaCl (incubated): 1 - 11% hemolysis
Reference values have not been established for patients who are <12 months of age.
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Performing Laboratory

Mayo Clinic Laboratories
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New York DOH Approval Status

Yes
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