Test Details
Mnemonic
QFTBI
Ordering Code
3000244
CPT-4 Code(s)
86480
Specimen Information
Collect
QuantiFERON® Collection Kit:
QFT-NIL Control - 1.0 mL whole blood (gray cap white ring*)
QFT-TB1 Antigen- 1.0 mL whole blood (green cap white ring*)
QFT-TB2 Antigen- 1.0 mL whole blood (yellow cap white ring*)
QFT-Mitogen Control - 1.0 mL whole blood (purple cap white ring*)
* High Altitude Collection Kit tubes have caps with a yellow ring.
QFT-NIL Control - 1.0 mL whole blood (gray cap white ring*)
QFT-TB1 Antigen- 1.0 mL whole blood (green cap white ring*)
QFT-TB2 Antigen- 1.0 mL whole blood (yellow cap white ring*)
QFT-Mitogen Control - 1.0 mL whole blood (purple cap white ring*)
* High Altitude Collection Kit tubes have caps with a yellow ring.
Specimen Preparation
1 - For each patient, collect 1.0 mL of blood by venipuncture directly into each of the four QuantiFERON® - TB Gold Plus blood collection tubes. The black mark on the side of the tube indicates the 1.0 mL fill volume. Under or overfilling tubes my produce erroneous results. If a "butterfly needle" is being used to collect blood, a "purge" tube should be used to ensure that the tubing is filled with blood prior to the QFT-Plus Blood Collection Tubes being used.
2- Shake tubes 10 times immediately after filling the tube to ensure the entire surface of the tube is coated with blood to solubilize antigens in the tube walls. Overly energetic shaking may cause gel disruption and could lead to aberrant results.
3- Incubate all four (4) tubes upright at 37 °C for 16 - 24 hours. Incubation must commence within 16 hours of collections. DO NOT REFRIGERATE or FREEZE WHOLE BLOOD SAMPLES.
4 - After incubation, centrifuge 4 collection tubes for 15 minutes at 2000 - 3000 RCF (g). Spun samples are stable for 28 days at 2-8 °C (refrigerated). Transport refrigerated samples to Warde Medical Laboratory in the original collection tubes.
2- Shake tubes 10 times immediately after filling the tube to ensure the entire surface of the tube is coated with blood to solubilize antigens in the tube walls. Overly energetic shaking may cause gel disruption and could lead to aberrant results.
3- Incubate all four (4) tubes upright at 37 °C for 16 - 24 hours. Incubation must commence within 16 hours of collections. DO NOT REFRIGERATE or FREEZE WHOLE BLOOD SAMPLES.
4 - After incubation, centrifuge 4 collection tubes for 15 minutes at 2000 - 3000 RCF (g). Spun samples are stable for 28 days at 2-8 °C (refrigerated). Transport refrigerated samples to Warde Medical Laboratory in the original collection tubes.
Minimum Volume
QFT-NIL Control - 0.8 mL whole blood (gray cap white ring*)
QFT-TB1 Antigen- 0.8 mL whole blood (green cap white ring*)
QFT-TB2 Antigen- 0.8 mL whole blood (yellow cap white ring*)
QFT-Mitogen Control - 0.8 mL whole blood (purple cap white ring*)
QFT-TB1 Antigen- 0.8 mL whole blood (green cap white ring*)
QFT-TB2 Antigen- 0.8 mL whole blood (yellow cap white ring*)
QFT-Mitogen Control - 0.8 mL whole blood (purple cap white ring*)
Transport Temperature
Refrigerated
Rejection Criteria
Frozen tubes, unspun collection tubes, tubes with fill volumes below or above the black mark, specimens not in QuantiFERON® collection tubes, samples not incubated, or samples incubated in a water bath or heat block.
Stability
Incubated and spun samples :
Room temperature: Unacceptable
Refrigerated: 28 days
Frozen: Unacceptable
Room temperature: Unacceptable
Refrigerated: 28 days
Frozen: Unacceptable
Methodology
Chemiluminescence Immunoassay (CLIA)
Reference Range
Negative
Performing Laboratory
Warde Medical Laboratory
New York DOH Approval Status
Yes