Test Details
Mnemonic
RVPCR
Ordering Code
3091910
CPT-4 Code(s)
87634
Specimen Information
Collect
Variable specimen types
Specimen Preparation
Specimen source is required.
Throat plus NP swab in viral transport medium
If sending one sample for RVPCR and COVW (COVID testing) please send a minimum of 1.5 mL.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Bronchoalveolar lavage/wash in an IATA-approved sterile screw capped plastic container. 1.0 mL (0.5 mL minimum).
Sputum undiluted in an IATA-approved sterile screw capped plastic container. 1.0 mL (0.5 mL minimum).
Nasal Aspirates in vacuum trap. 1.0 mL (0.5 mL minimum).
Nasal Washes in an IATA-approved sterile screw capped plastic container. 1.0 mL (0.5 mL minimum).
NP/OP swab in Phosphate Buffered saline or sterile saline.
Throat plus NP swab in viral transport medium
If sending one sample for RVPCR and COVW (COVID testing) please send a minimum of 1.5 mL.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Bronchoalveolar lavage/wash in an IATA-approved sterile screw capped plastic container. 1.0 mL (0.5 mL minimum).
Sputum undiluted in an IATA-approved sterile screw capped plastic container. 1.0 mL (0.5 mL minimum).
Nasal Aspirates in vacuum trap. 1.0 mL (0.5 mL minimum).
Nasal Washes in an IATA-approved sterile screw capped plastic container. 1.0 mL (0.5 mL minimum).
NP/OP swab in Phosphate Buffered saline or sterile saline.
Alternate Specimen
The laboratory Director or Supervisor must approve testing of specimens other than those listed.
Minimum Volume
0.5 mL
Transport Temperature
Refrigerated
Rejection Criteria
Specimens in Amplicor, EIA, Gen-Probe, or ProbeTec transport media.
Specimens in bacteriological blood culture media.
Dry swabs, calcium alginate swabs, and swabs in gel transports.
Culturettes older than 24 hours.
Specimens received in non-sterile or leaking containers.
Whole blood
Serum
Plasma
Specimens in bacteriological blood culture media.
Dry swabs, calcium alginate swabs, and swabs in gel transports.
Culturettes older than 24 hours.
Specimens received in non-sterile or leaking containers.
Whole blood
Serum
Plasma
Stability
Room temperature: 3 days
Refrigerated: 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months
Refrigerated: 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months
Methodology
Reverse transcription of conserved regions of the respiratory syncytial virus (RSV) RNA genome, polymerase chain reaction amplification, and real-time detection. This assay detects the presence of a conserved region of the RNA polymerase gene from RSV A and RSV B. This method cannot distinguish the A and B subtypes. The analytical sensitivity of this assay is 100 copies/mL. A negative result does not rule out infection.
Reference Range
Negative
Performing Laboratory
Warde Medical Laboratory
New York DOH Approval Status
No