Test Details
Mnemonic
RNPCR
Ordering Code
3092220
CPT-4 Code(s)
87798
Specimen Information
Collect
Variable specimen types
Specimen Preparation
Specimen source required.
NP and throat swabs in viral transport medium.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Bronchoalveolar lavage/wash in an IATA-approved sterile screw capped plastic container. 1.0 mL (0.5 mL)
Sputum undiluted in an IATA-approved sterile screw capped plastic container. 1.0 mL (0.5 mL minimum)
Nasal aspirates in vacuum trap. 1.0 mL (0.5 mL minimum)
Nasal washes in an IATA-approved sterile screw capped plastic container 1.0 mL (0.5 mL minimum)
NP and throat swabs in viral transport medium.
Swabs in culturettes must be transferred to viral transport within 24 hours of collection.
Bronchoalveolar lavage/wash in an IATA-approved sterile screw capped plastic container. 1.0 mL (0.5 mL)
Sputum undiluted in an IATA-approved sterile screw capped plastic container. 1.0 mL (0.5 mL minimum)
Nasal aspirates in vacuum trap. 1.0 mL (0.5 mL minimum)
Nasal washes in an IATA-approved sterile screw capped plastic container 1.0 mL (0.5 mL minimum)
Alternate Specimen
The Laboratory Director or Supervisor must approve testing of specimens other than those listed.
Minimum Volume
0.5 mL
Transport Temperature
Refrigerated
Rejection Criteria
Specimens in Amplicor, EIA, Gen-Probe, or Probetec transport media
Specimens in bacteriological blood culture media
Dry swabs, calcium alginate swabs, and swabs in gel transports
Culturettes older than 24 hours
Specimens received in non-sterile or leaking containers
Whole blood
Serum
Plasma
Specimens in bacteriological blood culture media
Dry swabs, calcium alginate swabs, and swabs in gel transports
Culturettes older than 24 hours
Specimens received in non-sterile or leaking containers
Whole blood
Serum
Plasma
Stability
Room temperature: 3 days
Refrigerated: 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months
Refrigerated: 14 days
Frozen (-20°C): 14 days
Frozen (-70°C): 3 months
Methodology
This test utilizes a reverse transcriptase real-time PCR procedure to detect the 5'-nontranslated region of the rhinovirus genome. This procedure will detect most, but not all of the >200 rhinovirus types. This procedure cannot differentiate the individual rhinovirus types. This procedure is significantly more sensitive than culture for rhinovirus detection. The analytical sensitivity of this assay is 200 copies/mL. A negative result does not rule out infection.
Reference Range
Negative
Performing Laboratory
Warde Medical Laboratory
New York DOH Approval Status
No