Test Details
Mnemonic
VONWI
Ordering Code
3400356
CPT-4 Code(s)
81408, add 88235 if testing is performed on amniotic fluid or chorionic villi at additional charge
Specimen Information
The following germline genetic tests from Quest require physician attestation that patient consent has been received if ordering medical facility is located in AK, DE,
FL, GA, IA, MA, MN, NV, NJ, NY, OR, SD or VT or test is performed in MA.
Collect
Lavender EDTA
Specimen Preparation
Draw blood in lavender EDTA tube. Send 5.0 mL whole blood.
Alternate Specimen
Yellow top (ACD), sodium heparin royal blue top,
Sodium heparin green top, amniotic fluid, chorionic villi collected in a sterile leak-proof
container, cultured cells collected in each of two sterile T-25 flasks.
Sodium heparin green top, amniotic fluid, chorionic villi collected in a sterile leak-proof
container, cultured cells collected in each of two sterile T-25 flasks.
Minimum Volume
Whole blood: 3.0 mL, Amniotic fluid: 10.0 mL, Chorionic villi: 10.0 mg,
Cultured cells: 75% confluent
Cultured cells: 75% confluent
Transport Temperature
Room temperature
Rejection Criteria
Plasma, Refrigerated specimens, Frozen specimens
Stability
Whole blood:
Room temperature: 14 days
Refrigerated: 14 days
Frozen: Unacceptable
Amniotic fluid, cultures cells, chorionic villi:
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Room temperature: 14 days
Refrigerated: 14 days
Frozen: Unacceptable
Amniotic fluid, cultures cells, chorionic villi:
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Methodology
Next Generation Sequencing
Reference Range
See report
Performing Laboratory
Quest
New York DOH Approval Status
Yes