EBVQN – “Epstein-Barr Virus DNA PCR, Quantitative”

Update to specimen stability, rejection criteria, methodology, reference range, and example report on website.

Rejection Criteria: Serum, heparinized plasma, whole blood, specimens submitted to repeated freeze-thaw cycles 

Stability:

Room temperature: 24 hours
Refrigerated: 5 days
Frozen (-20°C): 30 days
Frozen (-70°C): 6 months

Methodology: Polymerase Chain Reaction (PCR)
This test uses the polymerase chain reaction to amplify regions of the Epstein Barr Virus BLLF1 gene. Real-time detection and quantification are used to determine the viral concentration. The analytical measurement range is 50 to 200 million IU/mL (1.7 to 8.3 log (10) IU/mL). The qualitative limit of detection is 20 IU/mL (1.3 log (10) IU/mL).
Specimens reported as “DETECTED” but <50 IU/mL, contain detectable levels of EB Virus DNA but the viral load is below the limit of quantification. A “NOT DETECTED” result does not rule out infection.

Reference Range:

EBV Qualitative: Not Detected
EBV Quantitative: <50 IU/mL
Log EBV: <1.7 log(10) IU/mL