CADEP – “Cadmium Exposure Panel-OSHA”

Update to specimen requirements, stability, rejection criteria, methodology and reference range

Collect: Dark blue K2EDTA and minimum 40 mL urine

Specimen Preparation:

Cadmium, Blood: Draw blood in a dark blue K2EDTA. Send 3.0 mL whole blood in original collection tube or a blue-capped ARUP metal-free screw capped plastic vial.

Urine: Collect 40.0 mL random urine

Cadmium, Urine: Send 7.0 mL urine from original urine collection in a blue-capped ARUP metal-free screw capped plastic vial labeled “Cadmium”.

Creatinine, Urine: Send 2.0 mL urine from original urine collection in an screw capped plastic vial labeled “creatinine”.

B-2-Microglobulin, Urine: Send 3.0 mL urine aliquot from original urine collection, adjusted to pH 6 – 8 using 1M HCl or 5% NaOH, frozen in an screw capped plastic vial, labeled B2M. Freeze within one hour of collection.

Transport Temperature:

Blood: Refrigerated
Urine for Beta-2-Microglobulin: Frozen
Urine for Cadmium: Refrigerated
Urine for Creatinine: Refrigerated

Rejection Criteria:

Blood – Specimens collected in tubes other than Dark Blue K2EDTA or Royal Blue Sodium heparin.
Clotted specimens
Cadmium, Urine – Specimens not in blue-capped ARUP metal-free vials
Specimens containing blood or fecal material
Specimens collected within 72 hours after administration of iodinated or gadolinium-based contrast media

Stability:

Cadmium, Blood:
Room temperature: Indefinitely
Refrigerated: Indefinitely
Frozen: Unacceptable

Cadmium, Urine:
Room temperature: 7 days
Refrigerated: 14 days
Frozen: 1 year

Creatinine, Urine:
Room temperature: 24 hours
Refrigerated: 30 days
Frozen: 6 months

Beta-2-Microglobulin, Urine:
Room temperature: 8 hours
Refrigerated: 2 days
Frozen: 60 days

Methodology:

Quantitative Inductively Coupled Plasma – Mass
Spectrometry/Spectrophotometry/Chemiluminescent Immunoassay

Reference Range:

Beta-2-Microglobulin, ratio to CRT: 0-300 μg/g CRT
Beta-2-Microglobulin, Urine: 0-300 μg/L
Cadmium, Urine – per volume: <= 1.0 μg/L
Cadmium, Urine – ratio to CRT <= 3.0 μg/g CRT
Cadmium, Whole blood: <= 5.0 μg/L